Composite

Part:BBa_K4719014:Design

Designed by: Auguste Stankeviciute   Group: iGEM23_Vilnius-Lithuania   (2023-09-03)


AGM1-GFA1-GNA1-UAP1 operon for synthesis of bacterial cellulose-chitin copolymer


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal BglII site found at 1484
    Illegal BglII site found at 1583
    Illegal BglII site found at 3033
    Illegal BamHI site found at 4339
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2076
    Illegal EcoRI site found at 2574
    Illegal EcoRI site found at 4000
    Illegal EcoRI site found at 4929
    Illegal EcoRI site found at 5091
    Illegal XbaI site found at 3174
    Illegal SpeI site found at 37
    Illegal SpeI site found at 4867
    Illegal SpeI site found at 5755
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

.


Source

synthetic

References